New Delhi: The Income Tax Department has attached Rs 254 crore worth of “benami” equity of Madhya Pradesh Chief Minister Kamal Nath’s nephew Ratul Puri which he is stated to have allegedly received from a suspect in the VVIP chopper scam via shell companies, officials said on Tuesday.Ratul Puri, the chairman of Hindustan Powerprojects Pvt (HPP) Ltd, is already under scanner of the tax department and the Enforcement Directorate on charges of tax evasion and money laundering. Also Read – Uddhav bats for ‘Sena CM’Officials said a provisional order for attachment of the shares or non-cumulative compulsory convertible preference shares (CCPS) has been issued under section 24 (3) of the Prohibition of Benami Property Transactions Act by the Delhi-based office of the department. The funds were received as FDI (foreign direct investment) in Optima Infrastructure Pvt Ltd, a group concern of Moser Baer, a firm promoted by Ratul Puri’s father Deepak Puri, they said. The I-T department alleges that the investment of Rs 254 crore was generated through “over-invoicing of imports of solar panel’s by another group company, HEPCL, and was “facilitated through shell companies” of Dubai-based operator Rajiv Saxena, an accused in the AgustaWestland VVIP chopper scam case. Saxena was deported from Dubai by Indian agencies in January. Also Read – Farooq demands unconditional release of all detainees in J&KHe is under arrest by the ED in the chopper scam case, while Ratul Puri is being questioned. They said Ratul Puri is the beneficial owner of these benami equity shares and has been charged under the relevant law. These alleged benami and illegal funds, officials alleged, were generated by using “third party remittances” received by Saxena on behalf of Ratul Puri and were routed back to India via a Mauritius-based shell or dummy company– Rigel Power Limited. Further, they said quoting the order, the equity amount of Rs 254 crore introduced in Optima Infrastructure Pvt Ltd. was “immediately” transferred to another group company Unocon Infradevelopers Pvt Ltd., which in turn is holding investment of the group companies. The department has attached investments in Unocon Infradevelopers too as part of the probe, they said. Benami properties are those in which the real beneficiary is not the one in whose name the property has been purchased. Violators of the anti-benami law, enacted in 1988 but implemented from November 2016 by the Narendra Modi government, attract a rigorous imprisonment of up to seven years and fine up to 25 per cent of the fair market value of the property. The Act allows for prosecution of the beneficial owner, the benamidar, the abettor and the inducer to benami transactions.
An Air Canada flight from Toronto to Washington was forced to make an emergency landing Sunday evening after smoke was discovered in the cockpit.Flight 7618 was headed for Ronald Reagan airport when pilots discovered the smoke and diverted to Washington’s Dulles International Airport.Sky Regional, which operates Air Canada Express, says 63 passengers and four crew members were unharmed after exiting the plane on the tarmac at Dulles.Images posted online show emergency vehicles parked on the tarmac as passengers gather nearby.Andrew Trull, a spokesman for the Metropolitan Washington Airports Authority, says passengers were evacuated via an emergency slide and taken to the main terminal.He says there were no delays as a result of the incident, and all runways are now back up and running normally.
Notley, while fighting court challenges and the B.C. government over the project, pulled it back from the abyss after the pipeline’s owner started questioning whether it would ever get built. She got the federal government to buy it for $4.5 billionNo sooner had the champagne corks popped than the Federal Court of Appeal ruled in the fall that Ottawa hadn’t properly consulted with First Nation or examined the impact on marine life.The project is in limbo until those failings are addressed.“(The court ruling) means it has slowed down, but it is still alive and I think it is going to get done,” said Notley.The line has come to symbolize not only Alberta’s struggles to get resources to market but also frustration over a stubbornly slow rebound in the oil-based economy.There have been pro-pipeline demonstrations in recent weeks along with renewed fringe commentary for Alberta to leave Confederation. Notley has taken to sniping at Quebec for criticizing Alberta’s “dirty energy” and has joined critics who say Prime Minister Justin Trudeau’s federal government has failed _ offering sympathy but little else _ to sustain one of the core drivers of the national economy.Political analysts and recent opinion polls suggest Notley remains popular. But Jason Kenney and his United Conservatives will pick up the fight when the election is held, by law, sometime in March, April or May.Kenney suggests Notley is the author of her own misfortune by bringing in a carbon tax and working with Trudeau, who is making it harder to get approval for energy megaprojects.“I can understand perhaps why they tried to make it (work with Trudeau) but it has been a complete failure,” said Kenney in an interview. “Even the premier now seems to acknowledge that through the change in her tone. Too little, too late.” EDMONTON, A.B. – The year 2019 will decide whether Premier Rachel Notley’s NDP gets to finish the job of getting more oil to market or become the first party in provincial history to be one and done.“I know that the opposition wants to sort of revel in what they insist on hoping is defeat, but we’ve made more progress on getting a pipeline to tidewater than any other government has in the last 70 years,” Notley told The Canadian Press in a year-end interview.Notley will take voters to the polls in the spring after a 2018 that saw her tightly wrapped in the Gordian knot of the Trans Mountain pipeline expansion. It received a green light two years ago and would triple the amount of oil moving to the B.C. coast and from there overseas where it could fetch a better price. Kenney has a more combative approach and has promised to take the federal government to court over its carbon tax plan.Polls and political observers say the dominant election issues will be the economy, jobs, and who can help put food on the table.“If we had an election today, we’d have an overwhelming UCP majority,” said Calgary-based pollster Janet Brown.“Albertans are singularly focused on the economy and what’s necessary to get the economy moving. At this moment in time, they think Jason Kenney and the UCP would be more competent at managing those things.”The NDP has already signalled its attack lines by warning that a Kenney government would gut departmental budgets and threaten hard-won gains for women and the LGBTQ community.Brown said United Conservative numbers have remained strong since the party was formed in 2017 in a merger of the Wildrose and Progressive Conservative parties. So far, NDP attacks have not resonated.“They keep trying to throw stuff at Jason Kenney, trying to paint him as anti-LGBTQ, a bigot, a racist all sorts of things. My polling numbers just don’t suggest that any of that stuff is sticking. I’m just impressed not just where that UCP number is, but how unchanging it is.”Calgary political scientist Duane Bratt said Alberta may statistically be out of a recession but that’s not how people feel.“And when times are bad, you lash out at the incumbent government. That’s a simple fact.”Fighting hard to come up the middle is the Alberta Party under new leader and former Edmonton mayor Stephen Mandel.The party has long been a small-time player. It elected just one member in 2015 but added two more in floor-crossings. But it already has more than 50 candidates and expects to soon fill the full slate of 87.Mandel said the Alberta Party is the perfect middle-ground choice, because it combines the United Conservatives’ fiscal conservatism with the NDP’s socially progressive outlook.“People don’t like the polarization of the province,” he said. “They want to see more balance.”If a vote were held as early as the first week of March, a writ could be dropped as early as February. Political scientist Chaldeans Mensah said that’s when voters will really get engaged and fortunes could change quickly.“Right now the UCP is looking good, but I think it’s too early to conclude that the election is over,” said Mensah of MacEwan University in Edmonton.“The campaign and its dynamics will really tell the tale.”
MINNEAPOLIS — A federal judge in Minnesota has lifted a hold that had been placed on four lawsuits challenging the proposed PolyMet copper-nickel mine in northeastern Minnesota.Thursday’s decision by U.S. District Judge Joan Ericksen doesn’t list next steps, but her decision to lift the stay means the cases can proceed.The lawsuits filed by environmental groups seek to block a land swap that PolyMet needs to move forward.PolyMet wants to swap 6,650 acres of federal land in the Superior National Forest for a similar amount of private land.Ericksen put the cases on hold in March while Congress considered legislation that would have forced the land exchange. But that legislation didn’t become law.The environmental groups say the deal undervalues the federal land and violates other laws. PolyMet disputes that.The Associated Press
Under-Secretary-General for Management Angela Kane told a news conference at UN Headquarters that so far, 13 of the 192 Member States have paid in full all of the assessments that were due and payable as of today. These include assessments for the UN regular budget, as well as for its peacekeeping operations, for the International Criminal Tribunals for Rwanda and the former Yugoslavia and the Headquarters renovation project known as the Capital Master Plan.The countries that are paid in full are Australia, Azerbaijan, Canada, Democratic Republic of Congo, Denmark, Germany, Liechtenstein, Monaco, the Netherlands, Singapore, South Africa, Switzerland and Tanzania.Turning to the regular budget, Ms. Kane, who provided a snapshot of the UN’s current financial picture to the General Assembly on Tuesday, said that 119 Member States have paid their assessments in full, leaving 73 others who still have to do so. The unpaid assessed contributions to the regular budget amounted to $787 million, of which 88 per cent – or $691 million – is owed by a single Member State, the United States, she noted in response to a journalist’s question.Meanwhile, 9 per cent of the $787 million amount is owed by four countries – Mexico ($50 million), Chile ($9 million), Venezuela ($7 million) and Iran ($5 million) – and 3 per cent by the remaining 68 Member States. “There is a concentration among some Member States who have not paid their assessments but, on the other hand, we also are recognizing the current global economic and financial crisis that has made it difficult for some Member States to contribute on time and in full,” said the management chief.“Our work depends on the Member States supporting us financially and, therefore, it is critical for Member States, and particularly major contributors, to meet their financial obligations.”The UN’s regular budget is approved by the Assembly for a two-year period. Last December it approved a budget of $5.16 billion for the 2010-2011 biennium. Contributions are assessed on a scale based primarily on countries’ ability to pay. In 2000, the Assembly fixed a maximum of 22 per cent of the budget for any one contributor.As for the outstanding amount for peacekeeping operations, Ms. Kane said that it stood at $3.2 billion as of 5 October, about $1.3 billion more than at year’s end in 2009. In addition, the Organization’s debt to Member States that contributed troops and equipment to peacekeeping operations would be about $430 million at the end of 2010. This is less than the projection made in May and less than the $775 million outstanding at the end of 2009.On the $1.9 billion Capital Master Plan, she noted that $1.4 billion had been received so far, leaving $84 million still outstanding. 14 October 2010While noting that it has been a difficult year for many countries due to the global recession, a top United Nations official today urged all Member States that have not yet done so to meet their financial obligations to the world body so that it can continue to carry out its important work.
In this Saturday, Aug. 29, 2015 photo provided by Egypt’s state news agency MEAN, Eni CEO Claudio Descalzi, third left, and his delegation meet with Egyptian President Abdel-Fattah el-Sissi, center, and an Egyptian delegation, in Cairo, Egypt. The Italian energy company Eni SpA announced Sunday, Aug. 30, 2015, it has discovered a “supergiant” natural gas field off Egypt, describing it as the “largest-ever” found in the Mediterranean Sea. Eni said the discovery — made in its Zohr prospect “in the deep waters of Egypt” — could hold a potential 30 trillion cubic feet of gas over an area of 100 square kilometers (38.6 square miles). (MEAN via AP) by The Associated Press Posted Aug 30, 2015 7:35 am MDT Last Updated Aug 31, 2015 at 11:00 am MDT AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to RedditRedditShare to 電子郵件Email ROME – The Italian energy company Eni SpA announced Sunday it has discovered a “supergiant” natural gas field off Egypt, describing it as the “largest-ever” found in the Mediterranean Sea.The news came a day after Eni CEO Claudio Descalzi met in Cairo with Egyptian President Abdel-Fattah el-Sissi, the Egyptian leader’s office said.Eni said the discovery — made in its Zohr prospect “in the deep waters of Egypt” — could hold a potential 30 trillion cubic feet of gas over an area of 100 square kilometres (38.6 square miles).The discovery well is 190 kilometres (about 120 miles) from the Egyptian coast, and is at a depth of 1,450 metres ( 4,757 feet) in the Shorouk Block, the company said. The block is about 107 kilometres (66 miles) from Port Said.“Zohr is the largest gas discovery ever made in Egypt and in the Mediterranean Sea and could become one of the world’s largest natural gas finds,” Eni said in a statement. “The discovery, after its full development, will be able to ensure satisfying Egypt’s natural gas demand for decades.”“Eni will immediately appraise the field with the aim of accelerating a fast-track development of the discovery,” the energy company said.Descalzi was quoted by Eni as saying that the discovery reconfirms that “Egypt still has great potential” energy-wise. He said “important synergies with the existing infrastructures can be exploited, allowing us a fast production startup.”Eni has been in Egypt since 1954 through its subsidiary IEOC. It is the main hydrocarbon producer in Egypt, with a daily equity production of 200,000 barrels of oil equivalent, the company said.Egypt’s petroleum ministry said that the find was equal to about a third of Egypt’s current gas reserves.“The Egyptian government is very happy with this find,” ministry spokesman Hamdi Abdelaziz said, adding that the gas would be extracted for domestic consumption only.“We hope to become self-sufficient by 2020,” he added.The government cannot yet place a monetary value on the discovery, he said. Italian energy giant Eni says it has found largest-ever gas field in Mediterranean off Egypt
The UN Special Rapporteur on minorities, Fernand de Varennes, said Governments must live up to their international human rights obligations to protect and promote the rights of the Roma people.“There is a need for more action to combat hate speech, intolerance and racially motivated-violence,” Mr. de Varennes, in a statement on International Roma Day, also known as International Romani Day.He noted recent reports of intimidation, aggression and physical violence against Roma people in Europe, including on social media in recent years. “I note with grave concern how deeply entrenched social perceptions, bigotry and stereotypes about Roma minorities are used as a means to stir up hatred, leading to the perpetration of heinous acts against them,” the UN expert said.Pointing to inequalities, changing economic and political environments, and rising populism, he said many factors contribute to “a breeding group of expressions of hatred, exclusion, scapegoating and acts of violence” against the Roma.It is up to Governments to ensure that minority communities such as the Roma have the opportunities and respect they need, to succeed as productive and responsible members of society, said Mr. de Varennes.He added that strong commitment and resolute action are needed to effectively combat inflammatory and racist rhetoric and practices that “dehumanise, demonise and discriminate against Roma,” including how they are portrayed in the media, and to ensure equal access to justice and effective remedies.In his statement, the UN expert also paid homage to the “considerable contributions” which the Roma have made, including through their artistic and cultural heritage.
Mining excavator and haul truck major Liebherr has provided a market update on its existing ultraclass model, the T282C, as well as the TI274 which is in its final testing stages, and the all new T264.Based on the latest product launch, the T282C mining truck, Liebherr states that its sales forecast projection through 2015 looks solid. The group anticipates growth next year with demand for some 60 units in 2011. Going forward through 2015, a consistent demand level of 75 units per year for the T282C is expected.The key recent order is from Peabody Energy in Wyoming, and involves the delivery of 32 units by December 2011 with the potential for more thereafter. Growth markets include the high altitude copper projects emerging in South America. The Canadian oil sands market is also extremely strong for ultra-class trucks, and the first T282C will head there in 2011. In Brazil, Vale is now also an important customer.The company has also given an update on the TI274, first unveiled at Bauma in 2007. At present, Liebherr states that it is testing and validating design changes and updates to the concept truck. The TI design concept utilises the dump body as a structural member of the truck and thus has a significantly reduced frame weight. The dump body, unlike conventional style mining trucks, does not rest on the frame but on the hoist cylinders and rear suspensions only. By comparison, the TI274 is only 10 short tons heavier yet hauls 60 short tons greater payload than the leading competitor in the 240 short ton class of mining trucks. The next step in the development process which will involve moving the proof of concept truck from the Liebherr proving grounds to an actual mine site where its performance can be monitored in an actual production environment.A prototype of the latest truck development, the T264 truck, rated at 220t, will be sent to the proving ground as well. The T264 will have a standard 2,700hp Tier 2 engine and will be a good match for the Liebherr excavator product line.
Back in 2010, a developer called Kairosoft released Game Dev Story, a mobile game in which you assumed the role of the head of a game development studio, and created a plethora of video games from the very beginnings of the video game industry right up until the current generation. It’s a strategy game that’s light on strategy — you essentially just pick options out of a list and wait for timers to count down — but the game had a very charming combination of insider knowledge and tiered upgrades. Greenheart Games has released a different game in the style of Game Dev Story, dubbed Game Dev Tycoon, but where the gameplay may be similar, the studio’s method of deployment and DRM is far different — and much more amusing.Greenheart released two versions of its game: the first is the standard version you can buy from its website, the second is something of a social experiment. Greenheart intentionally uploaded a cracked version of its game to torrent sites, but modified a bit of the code. As a form of DRM, the version intentionally cracked by Greenheart includes piracy as one of the game mechanics. So, instead of developing a game and making money based on its quality and level of marketing, players will have to deal with their in-game video game being the target of in-game pirates.If the real-life pirates aren’t aware of the experiment, then they could easily think that piracy is canonically part of Game Dev Tycoon, as the message appears in the game like any other notification.However, it would seem that rather than piracy just being some kind of sustainable loss, your studio will eventually be put out of business due to the snowballing nature of the losses.A trip to the game’s official forums will show a number of users posting threads complaining about the piracy mechanic in the game, not realizing that they’re outing themselves as someone who downloaded the cracked version rather than purchased the game. Perhaps more amusingly, the people that did download that cracked version didn’t technically pirate the game, as the studio intentionally uploaded it for people to download for free.Sadly, Greenheart games said that only a little over 6% of people playing Game Dev Tycoon bought the game legitimately. Greenheart is offering a demo of the game, so you also wouldn’t “need” to pirate it to see if you like it enough to purchase it. The game costs just eight dollars, and has a version for Windows, OS X, and Linux.
Australian Greek Welfare Society (AGWS) in collaboration with the Greek Orthodox Community of Melbourne and Victoria (GOCMV) has commenced the implementation of the recently-funded project for the provision of services to newly-arrived Greek migrants.Via this project, AGWS will deliver community support programs, education and training, information and referral services to people seeking assistance and will endeavour to develop linkages across the regional areas where people newly arrived from Greece reside and work.The AGWS Settlement Services Project Officer will be based and provide services from the head office in Brunswick and one day a week, on Tuesdays, will be out-posted at the GOCMV to deliver education, training and community support programs and other innovative work.For further information on services please contact AGWS head office on 9388 9998. Facebook Twitter: @NeosKosmos Instagram
La tempête Jova fait deux morts au MexiqueMercredi, la tempête tropicale Jova est passée dans l’Etat de Jalisco à l’ouest du Mexique où elle a fait deux victimes : une mère et sa fille emportées par les pluies torrentielles après un glissement de terrain.Depuis dimanche, le Mexique s’était placé en alerte face à la menace de l’approche d’un ouragan de catégorie 2, Jova. Mercredi, au petit matin, le phénomène est arrivé sur le pays par les côtes pacifiques près de Punta Farallon. Il était accompagné de pluies torrentielles et de vents soufflant jusqu’à 160 kilomètres par heure qui ont frappé la côte de Melaque jusqu’à la station balnéaire de Manzanillo, à 800 kilomètres à l’ouest de Mexico.À lire aussiUn mystérieux tunnel souterrain découvert sous une pyramide au MexiqueSurvolant la côte, les intempéries ont causé des inondations, des effondrements de bâtiments, des chutes d’arbres et de panneaux publicitaires. Ainsi, dans le village de Melaque, une mère et sa fillette âgée de cinq ans ont trouvé la mort, emportées par les pluies diluviennes après un glissement de terrain, ont expliqué les autorités locales. Pour l’heure, plus de 4.500 sinistrés sont à déplorer. Néanmoins, son arrivée sur les terres a bien affaibli Jova, qui a été reclassé en tempête tropicale en fin de matinée par les météorologues. Le gouvernement a de ce fait annoncé que les Jeux panaméricains 2011 qui doivent s’ouvrir vendredi à Guadalajara, capitale du Jalisco, à 320 kilomètres de la côte, auront bien lieu. Les fortes pluies prévues ne devraient affecter que les compétitions de volley-ball de plage, à Puerto Vallarta, selon les organisateurs cités par romandie.com. Le 13 octobre 2011 à 09:13 • Maxime Lambert
Berklee, NYC Save Power Station Studio berklee-new-york-city-save-iconic-power-station-studio Email Berklee, New York City Save Iconic Power Station Studio Twitter Facebook News With help from New York City, Berklee plans to renovate the legendary Manhattan recording studio and keep its doors openNate HertweckGRAMMYs Sep 8, 2017 – 1:54 pm One of New York City’s most hallowed musical grounds, the iconic Power Station studio has received new life thanks to the efforts of Boston-based Berklee College of Music and some help from the city itself.Renamed Avatar Studios in 1996, Power Station boasts an impressive list of artists and albums recorded within its walls, including Bruce Springsteen, David Bowie, Madonna, Paul McCartney, Rolling Stones, and Norah Jones.According to Billboard, Berklee has been working for 18 months on a plan to avoid the studio’s impeding closure, likely to become condominiums, by finding a way to finance a purchase of the building in order to preserve it as a recording facility.The plan continues to develop, as Berklee successfully closed on the purchase of the building in August, and now the New York City’s Mayor’s office of media and entertainment, in partnership with the Economic Development Corporation, will invest $6 million toward renovations.This funding, which combined with philanthropic investments adds up to $20 million, will allow Berklee to preserve the studio as a commercial facility and use it as an educational resource for its students and local musicians.”We really want to make this a place where innovators are attracted there and are doing things and collaborating with artists and creators from the music scene. We want to make this into a hub of creative activity,” says Stephen Webber, dean of strategic initiatives at Berklee and executive director of BerkleeNYC. “We want this to be the premier studio, not just in New York, but in the world. If you’re recording multiple musicians at the same time, we want you to come here. We just really endeavor to make this into a place where great music can happen.”Renovations are expected to take 12 to 18 months and are set to begin in late 2017 or early 2018.Read More: New York City To Host 2018 GRAMMYs
The page includes photos and GIFs alluding to the allegations that he touched women inappropriately (which he responded to last month), as well as past policy positions, some unflattering quotes and a situation with a Ukrainian prosecutor.By comparison, Biden’s official campaign site for the 2020 US presidential election is pretty hard to find via Google — the easiest way is through his Facebook or Twitter pages.The site’s anonymous creator said via email that they’d only “realized the extent” of Biden’s actions after compiling the coverage.”I thought that it would be a good idea to create a website that was funny and easy to digest which showcased a lot of his actions,” they wrote, noting that they had to cut some GIFs and images “for space and flow.”The New York Times, which opted to name the site in its reporting, said June 29 that it was created by Patrick Mauldin, who makes “videos and other digital content for President Trump’s re-election campaign. Together with his brother Ryan, Mr. Mauldin also runs Vici Media Group, a Republican political consulting firm in Austin.” It takes a while for Google’s systems to learn whether a new site is a reliable source of information, said a spokesperson for the company, and the ranking of Biden’s campaign site is likely to change over time.The Biden campaign didn’t immediately respond to a request for comment.Originally published May 7, 5:07 a.m. PT.Updates, 8:13 a.m.: Adds comment from the site’s creator; 11:39 a.m.: Includes comment from Google. Update, July 1, 11:51 a.m.: Updated to reflect reporting by The New York Times. Democratic presidential hopeful Joe Biden might not find the parody site funny. Getty Images Googling “Joe Biden” won’t bring up the Democratic presidential hopeful’s official campaign page, but one of the top results looks a whole lot like it.The parody site appears under the name “Joe Biden for President 2020″ and appears pretty legit at a glance. However, a close look at the language and imagery reveals the intent to mock the former vice president, who announced 2020 White House run in April.”Uncle Joe is back and ready to take a hands-on approach to America’s problems! Joe Biden has a good feel for the American people and knows exactly what they really want deep down.” it reads. “He’s happy to open up and reveal himself to voters and will give a pounding to anybody who gets in his way!” Trump criticizes ‘censorship’ on social media, SpaceX… 1:23 Share your voice Google Comments Tags Politics Internet Services 7 Now playing: Watch this:
As snow melts, wildfire is becoming a threat. Red Flag warnings are in effect for areas of South Central and Interior Alaska, including Fairbanks, Delta Junction and Tok.Download AudioReceding snow has exposed grasses and other dead vegetation, and National Weather Service meteorologist John Lingas says persistent high pressure is allowing them to dry out quickly.“It [has] really inhibited substantial moisture from getting into it, so we get a little bit of cloud in,” Lingas said. “But the dry conditions near the surface persist and, as each day goes on, they just get a little drier and that’s prompted relative humidity at or around 15 percent here over the last couple of afternoons and we expect the same today.”Lingas says a weak system is expected to provide some relief for the Fairbanks area tomorrow, with a chance of a little rain, or even snow at higher elevations, before things warm up and dry out again later in the week. “High pressure over the Alaska Peninsula now is gonna get shoved northward into the Interior and it’s gonna grow and get stronger, so we are looking for even warmer temperatures at the end of the week and then a return also to dry conditions,” Lingas said.
(Phys.org)—Synthetic biology is a multi-disciplinary field that applies engineering techniques to biological systems. While the foundations of synthetic biology were laid in the 1990s with the burgeoning of genomics and automated sequencing, synthetic biology as a discipline, emerged in the early 2000s with the development of simple gene regulatory circuits. Since then, the field has grown beyond making simple circuits to developing diagnostic tools. More information: Shimyn Slomovic et al. Synthetic biology devices for in vitro and in vivo diagnostics, Proceedings of the National Academy of Sciences (2015). DOI: 10.1073/pnas.1508521112AbstractThere is a growing need to enhance our capabilities in medical and environmental diagnostics. Synthetic biologists have begun to focus their biomolecular engineering approaches toward this goal, offering promising results that could lead to the development of new classes of inexpensive, rapidly deployable diagnostics. Many conventional diagnostics rely on antibody-based platforms that, although exquisitely sensitive, are slow and costly to generate and cannot readily confront rapidly emerging pathogens or be applied to orphan diseases. Synthetic biology, with its rational and short design-to-production cycles, has the potential to overcome many of these limitations. Synthetic biology devices, such as engineered gene circuits, bring new capabilities to molecular diagnostics, expanding the molecular detection palette, creating dynamic sensors, and untethering reactions from laboratory equipment. The field is also beginning to move toward in vivo diagnostics, which could provide near real-time surveillance of multiple pathological conditions. Here, we describe current efforts in synthetic biology, focusing on the translation of promising technologies into pragmatic diagnostic tools and platforms. Shimyn Slomovic, Keith Pardee, and James J. Collins of MIT and Harvard discuss the current trends in synthetic biology to make diagnostic tools, and what additional hurdles must be overcome before these methods can be applied in the field or in the clinical setting. Their article is part of a special hundredth anniversary series in the Proceedings of the National Academy of Sciences commemorating exceptional research published in the journal over the last century.The rational design of diagnostic tools in synthetic biology uses natural regulation and detection systems to design sensors. Ideally, these sensors are selective, sensitive, and provide some kind of output, such as fluorescence or a color change, when it has detected the target cell, environmental cue, or pathogen. Slomovic, Pardee, and Collins discuss three broad categories of synthetic biology-based diagnostic tools: whole-cell biosensing, in vitro diagnostics, and in vivo diagnostics.Whole-cell biosensingMicroorganisms can be employed as a kind of biosensor. One well-known example is photosynthetic cyanobacteria incorporated into hardware. The cyanobacteria are redox active. Electron transfer through the hardware will produce a current, but herbicides quench its redox activity. The lack of current serves as an indicator for the presence of herbicides in places such as natural water reservoirs. More recent research involves tailoring microorganisms using genetic engineering. Engineering methods allow scientists to design bacteria that are selective for a particular substance. For example, bacteria have been engineered to detect arsenic in water. These genetically engineered bacterial cells have been integrated into circuits and other mechanical components to produce a hybrid device. However, hybrid devices have lost popularity, giving way to plate readers which have a higher throughput. RNA-based biosensing can detect metabolites or RNA sequences that are specific to a particular disease. RNA biosensing works by releasing stalled RNA translation using a synthetic piece of RNA that contains a complementary portion of the target sequence. One of the difficulties with this technique, however, is that the RNA sensing portions are confined within the bacterial cell and are not accessible to the external environment. Paper-based synthetic gene networks could enable rapid detection of Ebola and other viruses In vitro diagnosticsBacteriophages are nature’s bacteria sensors. They are viruses that home in on particular bacteria and infect them. Engineered bacteriophages are relatively inexpensive to make and can be designed to provide output indicators, such as bioluminescence, when it has detected the presence of a particular bacterial strain. Bacteriophages have been used both as sensors and as alternatives to antibiotics. For example, phages allow scientists to detect the presence of bacteria associated with the bubonic plague in a human blood sample in a matter of hours.Paper-based diagnostics are a cheap, mobile method that shows promise for eventual field use. Using commercially available bacterial or mammalian transcription/translation systems, scientists are able to freeze-dry these systems on to paper or other porous materials. These systems target certain RNA sequences in a similar way to the RNA-based biosensing, mentioned above, but the circuitry is on paper rather than within the cell. The system becomes active when rehydrated, allowing the freeze-dried material to be easily transported. Recent tests with an Ebola virus disease detection system used color-changing enzymes for easy output. In vivo diagnosticsReal-time biosensing is attractive for monitoring changes in the organism’s internal environment. This can be done with engineered bacteria that are administered to the organism. One example is the use of engineered E. coli to monitor the gut microbiome. In mouse studies, E. coli. were engineered to detect and record exposure of the gut microbiome to a particular drug.Mammalian cells can also function as in vivo biosensors, and they have the added benefit of possibly serving as both the sensor and the therapy. These cells utilize synthetic gene networks that can either change or monitor the organism’s cellular environment. Additionally, cellular biosensing allows the synthetic circuit to be isolated from the environment but also able to interact with it.One way that mammalian cells have been used for diagnostics is to monitor the expression profiles of certain cells. Cells express, or “turn on”, some genes but not others. The particular set of genes that are turned on provides an expression profile. Some cancer cells, cells that have been infected by a virus, or cells whose immune system is malfunctioning (autoimmune) have different expression profiles from normal cells. These cells can be targeted and identified using engineered mammalian cells. This has the added benefit of potentially destroying the diseased cell either by turning on the cell’s self-destruct genes or by drug targeting.Unfortunately, the biggest difficulty with in vivo diagnostics is delivering the engineered cell or synthetic genetic construct to the right place. Slomovic, Pardee, and Collins point out that this is where the field of nanobiotechnology comes into play. Recent research shows that some nanoparticles will home in on certain cells types, and others have been looking at lipid-based vesicles as another possibility for targeted delivery.Slomovic, Pardee, and Collins point out that “success in synthetic biology will continue to be found at the interfaces between disciplines.” Synthetic biology, while still a relatively new field, draws from years of work in genetics, microbiology, engineering, and nanotechnology, and by combining these fields, researchers have been able to make formidable progress in biosensing and in vitro and in vivo diagnostics. Explore further This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Citation: Diagnostic tools using synthetic biology (2015, December 3) retrieved 18 August 2019 from https://phys.org/news/2015-12-diagnostic-tools-synthetic-biology.html Journal information: Proceedings of the National Academy of Sciences © 2015 Phys.org
Kolkata: Rudra Chatterjee, managing director, Luxmi Tea Company, took over as the president of Indian Chamber of Commerce (ICC) at the 90th Annual General Meeting of the body, which was held on Friday.Mayank Jalan, chairman & managing director, Keventer Agro Ltd., has been elected as the senior vice-president of ICC, whereas Vikash Agarwal, president, Rupa & Co. Ltd. has been elected as the vice-president.Rudra Chatterjee is also the chairman of Obeetee, India’s largest carpet company, apart from being the managing director of Luxmi Tea Company Pvt. Ltd., director of Makaibari, Biswa Bangla Marketing Ltd., Banglar Dairy, Manor & Mews, UK and Haldia Petrochemicals Ltd. Also Read – Rain batters Kolkata, cripples normal lifeHe has also worked in the New York office of Booz Allen Hamilton, a management consulting firm.Chatterjee had graduated from Columbia Business School. In addition, he is part of the faculty at IMI Kolkata and guest lecturer at IIM, Kolkata.Mayank Jalan, chairman and managing director, Keventer Agro Ltd. (KAL), joined the company in April 2004. A first-class degree holder in Mechanical Engineering from University College of London, Mayank also has a degree in Business Finance from the prestigious London School of Economics. Also Read – Speeding Jaguar crashes into Mercedes car in Kolkata, 2 pedestrians killedHe holds important offices across a number of industry forums and is also the Honorary Consul of Ireland in Kolkata.Vikash Agarwal is a Commerce Graduate from St Xavier’s College, Kolkata and has acquired a Diploma in Marketing from University of California, USA, before joining the company as President. He was invited to Champions of Change, an initiative by the NIITI Aayog, as part of a select group of entrepreneurs from around the country.Agarwal is also the director in Neo Metaliks Ltd. He is associated with Young President Organisation (YPO), acting as a board member of the Calcutta Chapter, The Bengal Rowing Club, Calcutta Swimming Club and Royal Calcutta Turf Club.
? pic.twitter.com/2xtG6KD6KV— Chad Johnson (@ochocinco) April 6, 2017 After the workout, Johnson challenged Odell Beckham to get locked:@OBJ_3 You next to get locked up too after I’m done with AB, 20 routes in bump-n-run you’ll catch 3‼️ ?— Chad Johnson (@ochocinco) April 3, 2017 I’m on his a** and I don’t play DB‼️ @AntonioBrown pic.twitter.com/gz4LvXuwGr— Chad Johnson (@ochocinco) April 4, 2017 I’m on his a** and I don’t play DB‼️ @AntonioBrown pic.twitter.com/gz4LvXuwGr— Chad Johnson (@ochocinco) April 4, 2017 Beckham will need some more convincing:@ochocinco listen. No lol— Odell Beckham Jr (@OBJ_3) April 4, 2017 My feet & hips, @AntonioBrown feet & hips. ??? pic.twitter.com/N2tWHgHI9P— Chad Johnson (@ochocinco) April 6, 2017 Talk that talk‼️ pic.twitter.com/j4q10MLz63— Chad Johnson (@ochocinco) April 6, 2017 Chad Johnson is trying his hand at defensive back, and issued a challenge to Antonio Brown on Twitter for the two to battle it out in some 1-on-1 passing drills.Brown accepted, and the two met a Florida football field to go at it over the course of two days.Johnson represented himself well, considering he was facing off against arguably the best wide out in the NFL and has never played the position. He broke up some passes and there’s no video of him getting completely roasted. Obviously, Brown caught some balls, he’s Antonio Brown. To be fair to the Steelers’ All-Pro, some of those passes weren’t exactly Big Ben level quality.Watch the videos, they do have some explicit language. Brown did not post a Facebook Live reaction in the locker room after the work out:? @AntonioBrown pic.twitter.com/bKaB8eXtoU— Chad Johnson (@ochocinco) April 4, 2017 Competition Competitive Consistency pic.twitter.com/di8zjFm7PA— Antonio Brown (@AntonioBrown) April 6, 2017
SANTOS, Brazil — Assistant coach Paulo Wanchope on Monday said Costa Rica have more surprises in store for heavyweights Italy and England after their shock defeat of Uruguay.The ex-Premier League star — and arguably the best player to ever play for Costa Rica — backed the underrated Central Americans to “keep surprising the world” after they floored Uruguay 3-1 to raise hopes of escaping from a tough Group D.“It’s a big surprise for the world — everyone expected either Uruguay or Italy or England to go through,” Wanchope told reporters after a training session at Santos Stadium.“For us, it wasn’t a surprise because we know the great talent that we have. We will keep working and keep surprising the world.”Goals from Arsenal’s Joel Campbell, Óscar Duarte and Marco Ureña downed Uruguay on Saturday and put European giants England and Italy on notice.And Wanchope, a hit at Derby Country, West Ham and Manchester City during his extended stint in England, said his side are ready for anything the high-profile Azzurri and Three Lions can throw at them.“We’re not surprised at anything. We’ve been looking at England for so long,” said Wanchope, when asked what he made of the attack-minded team that went down, 2-1, to Italy.“For me, especially, I do like the way they play, I do like the talent of [Adam] Lallana, [Dean] Sturridge, [Wayne] Rooney, [Steven] Gerrard. They’re very good going forward and they have good, solid defenders.”He said he expected England to improve in their second game against Uruguay, potentially setting up a crunch final group match when they face the Costa Ricans next week.“I think they could do better against Uruguay, I really do think that. But we will concentrate on our game and we will see what we can do against England in the last game,” said Wanchope.Costa Rica finished the Uruguay game with no yellow cards and no new injuries, meaning they will be at full strength for Friday’s game with Italy.Goalkeeper Keylor Navas said the team making detailed preparations to face an Italian team that includes the masterful Andrea Pirlo and Mario Balotelli, who scored the winner against England.“The next game is going to be very important for us,” Navas said. “We’re going to have to do everything right.”He added that he hoped Italian Gianluigi Buffon, one of his personal goalkeeping heroes, would recover from injury. Facebook Comments Related posts:Are you ready for Costa Rica vs. the Netherlands? 32 fascinating facts about Tico football Costa Rica’s last hurrah in historic World Cup run What I’ll tell my daughter about La Sele, 2014 Argentine Ricardo ‘El Tigre’ Gareca likely will be Costa Rica’s next national football coach
Arizona families, Arizona farms: working to produce high-quality milk Sponsored Stories Arizona families, Arizona farms: providing the local community with responsibly produced dairy Ibrahim and like-minded activists denied those in Kulikove Pole had any connection to subversive activities.“The blasts, these are likely the work of solo operators,” Ibrahim said. “They imagine that they are doing something that will benefit us somehow, whereas in fact they are only doing us harm.”When not patrolling the streets or trying to apprehend petty criminals, the Ukrainian patriotic groups also indulge in low-level intimidation of anti-government, pro-Russian groups.A press conference in Odessa last week given by the lawyer of a jailed Kulikove Pole activist was mobbed by a group of muscled men, who heckled and laughed throughout the proceedings. One man seen in the room was a leading figure in Panevsky’s organization.Nadezhda Melnichenko, a reporter with Odessa-based news site Timer, whose editorial line is strongly critical of the current government, said such petty harassment is commonplace.“There is nobody here for them to physically attack, but the appearance of young men with powerful bodies has a certain intimidating effect,” Melnichenko said outside the press conference hall.Panevsky argued that with the country fighting a war against what most Ukrainians believe to be an external aggressor — Russia — there is no room for squeamishness about going after those perceived to be a threat. New Valley school lets students pick career-path academies “It’s very important to look after every small piece of land, every millimeter of Ukraine,” Panevsky said. “We can’t allow anybody to denigrate or destroy our national values.”Copyright © The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed. Odessa lies more than 500 kilometers (300 miles) west of the front line in east Ukraine, where government troops are mired in a war of attrition against Russian-backed separatists. Still, anxiety about a second front emerging here is fueled by regular news of arrests of people suspected of plotting militant anti-government activities.Panevsky says authorities aren’t doing their job and units like his are stepping in. “The fight against separatism should be carried out by the security services, which are completely corrupt and compromised,” he said.His organization comprises 300 active volunteers, but that he can draw on hundreds more in the event of mass unrest, Panevsky said. The hardcore members of the unit gather for light arms training at a camp outside Odessa at the weekend.The legal status of such self-defense units is unclear, and some in the city speculate that they may be set on a collision course with authorities nervous about the presence of unaccountable armed gangs.By Panevsky’s own admission, his unit’s activities clearly cross into legally questionable territory. While giving a tour of his group’s headquarters, he showed a dank basement room where he said detained people are sometimes kept. Pressed about the whereabouts of the captured pro-Russian activist, Panevsky demurred and said the man would soon be released. He quickly changed the subject, instructing underlings to organize a honey-trap drug purchase to catch a small-time pusher. It was unclear whether the suspected separatist agitator was in fact detained.Odessa has not been spared its share of unrest. The city tipped into bloodshed in May last year, when clashes between pro-unity and pro-Russian activists culminated in 48 deaths. The bulk of those deaths occurred after the pro-Russian crowd was cornered in a trade union building that was later set alight.Those events had an almost instantaneous chilling effect on the activities of Russia-leaning activists. Those who agree to speak publicly are wary about labels that they fear could attract attention from authorities.“When somebody says that I am pro-Russian and they are pro-Ukrainian, it feels as though they want to annul my Ukrainian citizenship,” said Maurice Ibrahim, a leading figure in the anti-government Kulikove Pole movement in Odessa. Kulikove Pole is named for the city square where the activists habitually congregate.Ibrahim’s background is a vivid illustration of the eclectic mix of interests encompassed by the movement that militates for Ukraine to develop closer ties with Russia instead of the West. He left behind the civil war in his native Lebanon in the mid-1980s to study in Odessa, and stayed. He became a Ukrainian citizen 10 years ago. Ex-FBI agent details raid on Phoenix body donation facility Mesa family survives lightning strike to home ODESSA, Ukraine (AP) — Striding through headquarters, Todor Panevsky gleefully announced that comrades in his self-defense unit had detained a pro-Russian separatist sympathizer in this picturesque port city.“We’ll ask him a few questions and then we’ll hand him over to the security services,” said Panevsky, a portly part-time opera singer who has refashioned himself as the commander of an armed patriotic vanguard against secessionist sentiment in Odessa. 4 sleep positions for men and what they mean Here’s how to repair and patch damaged drywall For Ibrahim, the appeal of the pro-Russian camp stems from his radical left-wing political views. Others in his movement cleave to more emotive issues of ethnic ties to Russia, language rights and the perceived notion — energetically propagated by Kremlin media — that the Ukrainian government is pursuing a far-right agenda.Citizens of Odessa predominantly speak Russian, the lingua franca that unites a city renowned for its rich and long history of ethnic diversity. The promotion of a loudly Ukrainian nationalistic message smarts with those Odessans who take pride in their distinctive identity.Moscow’s hand is seen behind much of the violent trouble that has hit Ukraine over the past year, including in Odessa.In April, the Security Service of Ukraine announced it had detained 10 people suspected of planning to set off explosions in the city. The security services said the group was found in possession of bomb-making equipment, guns and flak jackets. Officials claimed the men were being coordinated by the Russian special services, although it offered no evidence.Odessa’s regional branch of security services told The Associated Press it could divulge no information on people currently facing charges of separatist activities, but it did say that no Russian citizens have been arrested. In this photo taken on Thursday, June 25, 2015, members of a self-defense unit carry out exercise drills in a location outside Odessa, Ukraine. Odessa lies more than 500 kilometers (300 miles) west of the front line in east Ukraine, where government troops are mired in a war of attrition against Russian-backed separatists. (AP Photo/Sergei Chuzavkov)n 0 Comments Share Top Stories
Best Western enters Tokyo’s vibrant Machida districtBest Western® Hotels & Resorts has continued to expand its presence in Japan with the launch of a new hotel in the vibrant Machida district of Tokyo.Now open to guests, Best Western Rembrandt Hotel Tokyo Machida is located in the western part of the Tokyo metropolitan area, with excellent transport links – including extensive rail connectivity – to the city center and other parts of Japan.For example, the popular Tokyo district of Shinjuku is only 35 minutes away via the Odakyu Line, and it takes just 20 minutes to travel to Yokohama on the JR Yokohama Line. Alternatively, guests can stay in Machida and explore the local area, which is blessed with multiple parks, museums and shopping malls.The hotel features a sleek, modern design and all rooms come equipped with contemporary amenities such as flat-screen TVs and complimentary Wi-Fi. Many of the rooms also feature panoramic windows that offer stunning views of the Machida skyline and the glittering lights of Tokyo beyond.Guests have a choice of dining options, with the Carillon Restaurant serving delicious local Japanese and international dishes, along with sumptuous buffets, and Long Fuang Restaurant specializing in exquisite Chinese cuisine.Best Western Rembrandt Hotel Tokyo Machida also features a range of options for meeting and event planners, including wedding services, function rooms and space for private parties.“I am pleased to welcome Best Western Rembrandt Hotel Tokyo Machida to our extensive collection of hotels in Japan,” commented Olivier Berrivin, Best Western Hotels & Resorts’ Managing Director of International Operations – Asia. “With its excellent location and connectivity, modern design and array of international amenities, I am confident it will become a popular destination for both business and leisure travelers.”Best Western operates a large portfolio of hotels all across Japan, from the snow-capped peaks of Hokkaido in the north to the beaches of Okinawa in the south. Best Western Rembrandt Hotel Tokyo Machida becomes the company’s second property in the Tokyo metropolitan area, following the Best Western Tokyo Nishikasai. Best Western Tokyo Machida Japan National Tourism Organisationdiscover JapanSource = Best Western